Web Portal cannot be accessed after update to version 8.1.5

Hello,


we have built a new development environment and updated there from version 8.0.5 to version 8.1.5.
Now we are in the process of installing and configuring the servers, but we have a problem with the WebServer that should display the Web Portal.
Here we get the following message from IIS:

500 - Internal server error.
There is a problem with the resource you are looking for, and it cannot be displayed.

We have compared the IIS settings of the previous web server with the IIS settings on the new web server and at first glance they look the same.

When we try to publish the WebProject in WebDesigner, the old and the new server appears under Edit - Publish all - Debug.
If we call Edit - Publish all - Release, only our previous (old) server appears here.
Can this be a problem?

Is there anything else that needs to be compiled here? Or what else could we check?
Unfortunately, our external web developer is currently away, so we can't wait that long for him to come back.

Thank you for any further advice on what else we should check.

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